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Welcome
Welcome
I am a Postdoctoral Researcher at the University of Minnesota (Selmecki Lab) specializing in genetics, bioinformatics, and DNA repair. My work integrates molecular biology with computational biology to study how antifungal treatment leads to genomic instability and how genomic instability leads to antifungal resistance.
Previously, as a PhD candidate at the University of Iowa , I investigated the mutagenic consequences of Break-Induced Replication (BIR). My research uncovered that BIR generates mutagenic single-stranded DNA not only on the lagging strand but also on the leading strand template (D-BTS). I further showed that similar error-prone pathways operate during meiosis and are capable of creating mutation clusters.
My technical expertise includes developing custom bioinformatic workflows for Next-Generation Sequencing (NGS) analysis. I created the MMBSearch pipeline for annotation of Microhomology-Mediated BIR (MMBIR) signatures in TCGA cancer datasets and MayoMate to find, visualize, quantify and correlate meiotic recombination events with ssDNA tracts by utilizing WGS data.
My Resume
My Resume
For more details on my experience and publications, please download my CV. I look forward to connecting with you regarding potential collaborations
JT Resume Fall 2024.pdfPublications
Twarowski, J.M., Kramara, J., Seuferer, G., Jiman, J., Malkova, A. Break-induced replication forms long hypermutable single-strand (ss) DNA during meiosis. Nature Communications (in review)
Liu, L., Lee, R.S., Twarowski, J.M., Wells, J., Jiman, J., Stefen, J., Lobachev, K., Malkova, A. (2025) Genome-wide screen reveals dependence of break induced replication on several distinct checkpoints. Nature Communications
Lee, R.S., Twarowski, J.M., Malkova, A. (2024) Stressed? Break-Induced Replication comes to the rescue! DNA Repair 10.1016/j.dnarep.2024.103759 article link
Lee, R.S., Geronimo, C., Twarowski, J.M., Malkova, A., Zakian, V., (2023). Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase. PloS Genetics 10.1371/journal.pgen.1010853 article link
Deshpande M, Paniza T, Jalloul N, Nanjangud G, Twarowski J, Koren A, Zaninovic N, Zhan Q, Chadalavada K, Malkova A, Khiabanian H, Madireddy A, Rosenwaks Z, Gerhardt J. (2022). Error-prone repair of stalled replication forks drives mutagenesis and loss of heterozygosity in haploinsufficient BRCA1 cells. Molecular Cell 10.1016/j.molcel.2022.08.017 article link
Osia*, B., Twarowski*, J., Jackson, T., Lobachev, K., Liu, L., Malkova, A. (2022). Migrating bubble synthesis promotes mutagenesis through lesions in its template. Nucleic Acid Research 50-12, 6870–6889. (*First Co-Authors) article link
Liu, L., Yan, Z., Osia, B., Twarowski, J., Sun, L., Kramara, J., Lee, R., Kumar, S., Elango, R., Li, H., Dang, W., Ira, G., Malkova, A. (2021). Tracking break-induced replication shows that it stalls at roadblocks. Nature 590, 655–659 article link
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